I've laid out a stripline transmitter for 100 MHz, the transmission line effects are substantial enough that you can turn circuit traces into coils and resonant circuits easily enough. The fact that it in the analog domain and that this is digital is the big difference but during the rise and fall of the clock those effects are very much present.
Interestingly if you are doing a standard height four layer stack up with something like signal-power-ground-signal your stripline is much closer to the plane and default trace widths have a natural impedance much closer to what you want. On two layer even if most of one side is a ground plane the distance from trace to plane leads to impedance much further out of spec. I haven't had to do any sort of impedance controlled traces yet luckily on a two layer where standard 1.6mm PCBs mean something crazy like 3mm trace widths to hit 50 ohms characteristic impedance at ~100-150MHz.
I think about 150mm is the furthest I've routed non-terminated 100MHz uSD lines and I've been lucky enough so far to get away with the only real design goal being all trace lengths within +/- 5% of the clock line, even with the trace lines having a couple hundred ohms of Z when the spec calls for 50. I do wonder things like if my error rate went from once per 20 years to once per 5 years type of thing. I'm also guessing the error rate follows some sort of inverse S-curve of probability.
